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determination of auxin concentration in plant growth

Current analytical methods for plant auxin …

Current analytical methods for plant auxin quantification – A review One of the main obstacles to auxin quantification is the low endogenous concentration of analyte present in plant samples. E. CarrilhoDirect determination of plant-growth related metabolites by capillary electrophoresis with spectrophotometric UV detection. J. Braz

Auxin metabolism rates and implications for plant …

2016-12-22 · Introduction. The plant hormone auxin plays a role in virtually every aspect of plant growth and development (Davies, 2004).It is transported cell-to-cell via several families of auxin-specific protein carriers, most famously the PIN family of auxin efflux carriers (Zazimalova et al., 2010).Auxin transport has been the subject of hundreds of studies, and is perhaps the best-characterized

Enhancing auxin accumulation in maize root tips …

(c) Auxin concentration of the leaf, coleoptilar node, LRZ (the 1.0–1.5 cm segment with lateral root outgrowth) and root tips of ZmPIN1a transgenic and WT lines cultured in nutrient solution for eight days. The boxes showed the regions for auxin determination. (d) Relative auxin concentration in different parts of maize seedlings.


 · Inhibition of growth is widespread in the plant kingdom, Just as the promotion of growth is controlled by the growth hormones, comprising principally the group known as the auxins, so also inhibitions are, in many cases at least, due to the action of auxins. The application of very high concentrations of auxin inhibits the growth of shoots

A High-Resolution Map of Auxin Distribution in the

2017-1-22 · The gradient and dynamic flow of the plant growth regulator auxin (indole-3-acetic acid [IAA]) in shoot and root apices is thought to be a critical factor in determining meristematic activity (i.e., the maintenance of stem cell niches and the regulated development of organ primordia) (reviewed in

Plant hormone

2019-10-11 · Plant hormones (also known as phytohormones) are signal molecules produced within plants, that occur in extremely low concentrations.Plant hormones control all aspects of growth and development, from embryogenesis, the regulation of organ size, pathogen defense, stress tolerance and through to reproductive development. Unlike in animals (in which hormone production is restricted to …

Auxin Control of Root Development

2017-1-1 · Auxin Control of Root Development. Paul Overvoorde, 1 Hidehiro Fukaki, 2 and Tom Beeckman 3, 4 the bimodal effect of auxin concentration on primary root length, Indole-3-butyric acid in plant growth and development. Plant Growth Regulation 32: 219–230; Malamy JE 2009.

Auxin Concentration/Growth Relationship for Avena

Abstract. A biphasic auxin dose-response curve has been obtained for indole-acetic acid (IAA)-stimulated growth of subapical sections of coleoptiles from totally dark-grown oats (Avena sativa L. cv Lodi).The curve for growth at 6 h is composed of a log-linear phase and a …

Utilization of the Plant Hormone Indole-3-Acetic Acid …

We have isolated from plant surfaces several bacteria with the ability to catabolize indole-3-acetic acid (IAA). One of them, isolate 1290, was able to utilize IAA as a sole source of carbon, nitrogen, and energy. The strain was identified by its 16S rRNA sequence as Pseudomonas putida . Activity of the enzyme catechol 1,2-dioxygenase was induced during growth on IAA, suggesting that catechol

Effects of selenium at different concentrations on root

2018-8-14 · Effects of selenium at different concentrations on root morphology, auxin content and auxin-related gene expression in tobacco LUO Yong 1, LIU Shengbo 2, ZHANG Tao 3, WANG Lizhi 1, XUAN Dongdong 1, XU Dihan 1, SHAO Huifang 1, JIA

Indole-3-Acetic Acid (IAA), and Auxin in Plants | Plant

2019-8-29 · ADVERTISEMENTS: In this article we will discuss about Indole-3-Acetic Acid (IAA) and Auxin in Plants. Natural and Synthetic Auxin: The main auxin in plants is indole-3-acetic acid (IAA Fig. 20-2). Additionally, several other naturraly occurring indole derivatives are reported to express auxin activity, including indole-3ethanol, indole-3-acetaldehyde, and indole-3-acetonitrile.

Development and Validation of a Reversed-Phase …

2012-1-30 · Auxins are a group of ubiquitous and vital plant phytohormones regulating plant growth and development [1, 2] and participating in plant response to biotic [3, 4] and abiotic stimuli .Although there are several naturally occurring compounds comprising auxin activity, indole-3-acetic acid (IAA) is the most abundant auxin .

Auxins and the inhibition of plant growth

Auxins and the inhibition of plant growth. Whether or not these phenomena of very high auxin concentration have any bearing upon normal growth inhibitions is still not clear. They may play a

() Regulation of Root Growth by Plant …

Regulation of Root Growth by Plant Hormones—Roles for Auxin and Gibberellin Article ( Available) in Critical Reviews in Plant Sciences 24(4):249-265 · July 2005 with 3,969 Reads

Phenotypical and molecular responses of Arabidopsis

known mechanism of direct plant growth promotion is phytosti-mulation, that is, the production of a substance (mostly phyto-hormones) that stimulates the growth of plants in the absence of pathogens (Lugtenberg & Kamilova, 2009; Spaepen etal., 2009). The best documented case of phytostimulation is the bacterial production of the plant hormone

The Yin-Yang of Hormones: Cytokinin and Auxin

The phytohormones auxin and cytokinin interact to regulate many plant growth and developmental processes. Elements involved in the biosynthesis, inactivation, transport, perception, and signaling of these hormones have been elucidated, revealing the variety of mechanisms by which signal output from these pathways can be regulated. Recent studies shed light on how these hormones interact with


2019-1-4 · The arrival of auxin in the cytosol initiates such immediate responses as changes in the concentration of and movement of ions in and out of the cell; reduction in the redistribution of PIN proteins. Some of the direct effects of auxin may be mediated by its binding to a cell-surface receptor designated ABP1 ("Auxin-binding protein 1").


2019-10-9 · Auxin induces shoot apical dominance; the axillary buds are inhibited by auxin, as a high concentration of auxin directly stimulates ethylene synthesis in axillary buds, causing inhibition of their growth and potentiation of apical dominance. When the apex of the plant is removed, the inhibitory effect is removed and the growth of lateral buds

Auxin: Regulation, Action, and Interaction | Annals of

As a critical plant hormone, auxin modulates such diverse processes as tropic responses to light and gravity, general root and shoot architecture, organ patterning, vascular development and growth in tissue culture (Davies, 1995). The importance of auxin for human sustenance is both vital and readily apparent: auxin is required for plant growth.

The Effects of High Steady State Auxin Levels on Root …

2016-5-5 · The long-standing Acid Growth Theory of plant cell elongation posits that auxin promotes cell elongation by stimulating cell wall acidification and thus expansin action. To date, the paucity of pertinent genetic materials has precluded thorough analysis of the importance of this concept in roots

Protocol: High-throughput and quantitative assays of …

 · The plant hormone auxin, indole-3-acetic acid (IAA), plays important roles in plant growth and development. The signaling response to IAA is largely dependent on the local concentration of IAA, and this concentration is regulated by multiple mechanisms in plants. Therefore, the precise quantification of local IAA concentration provides insights into the regulation of IAA and its biological roles.

Auxin-Induced Ethylene Triggers Abscisic Acid …

Plant growth and development is regulated by hormones, of which auxins are one of the classical five types, together with ethylene, gibberellins, (+)-abscisic acid (ABA), and cytokinins (Kende and Zee-vaart, 1997; Taiz and Zeiger, 1998).In cooperation with the other hormones, auxins, with indole-3-acetic acid (IAA) as the principal auxin in higher plants, stimulate fundamental processes such


2017-3-6 · sucrose in the growth medium enhanced the auxin production for all isolates except AUX142. The AUX36, AUX53 and AUX137, produced highest concentration of auxin at 0.1% peptone. Seed germination experiments using surface sterilized seeds of T. aestivum revealed that all isolates could increase root length of seedlings as compared to control.

Auxin-mediated growth of rice in cadmium-contaminated …

growth, metabolism, or other physiological activities, and they can alleviate the toxic effects of heavy metals on plant growth. Auxin is the most abundant natural growth hormone, controlling apical dominance, tropism, elongation of shoots, and initiation of roots in plants. However, cadmium stress causes the degradation of indigenous auxin.

The Effects of High Steady State Auxin Levels

The long-standing Acid Growth Theory of plant cell elongation posits that auxin promotes cell elongation by stimulating cell wall acidification and thus expansin action. To date, the paucity of pertinent genetic materials has precluded thorough analysis of the importance of this concept in roots. The recent isolation of mutants of the model grass species Brachypodium distachyon with

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